Composite

Part:BBa_K1913028:Design

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)


Wild type ptet-FixK2 hybrid promoter with mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 698
    Illegal AgeI site found at 810
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to test wild type ptet-FixK2 hybrid promoter,we put reporter mRFP under this promoter and co-transformed with light sensor BBa_K1913034. And mRFP fluorescence value was tested so that the intensity of transcription of this promoter could be quantified.



Source

Genomic sequence of Bradyrhizobium japonicum, iGEM part registry.

References